Injection of cdc42T17N RNA at a high does causes short body axes in both control siblings and gpc4^−/− embryos, and injection of a subdose does not disrupt the formation of protrusions in Tg(sox17:GFP-gpc4) embryos. (A–D) Bright-field images of the indicated embryos injected with a high dose of cdc42T17N RNA (250 pg). Lines with double arrows indicate length of the posterior body axis; lines of the same color are equal in length. (E) Average posterior body length in embryos shown in A–D. Colors of the P values correspond to the embryos in which the posterior body is marked with lines of the same color. (F and G) Snapshots from confocal time-lapse imaging performed on Tg(sox17:GFP-gpc4) embryos injected with a subdose of cdc42T17N RNA (120 pg). (H) Total number and length of protrusions in each endodermal cell of the embryos indicated shown in F and G. The number of embryos, cells, and protrusions analyzed is indicated. Data are mean ± SEM. #, P > 0.05; ****, P < 0.0001; unpaired Student's t tests.