Synergistic C&E defects in mesoderm and endoderm of wnt5b and wnt11f2 mutants. (A–D′) foxa3 expression, as assessed by WISH. (A–D) Lateral view. (A′–D′) Dorsal view. Red dashed lines represent the length of the posterior body, and white lines indicate the width of the gut tube. All lines of the same type are equal in length. (E–H′) Expression of hgg1 (arrowheads), dlx3, krox20, and ntl (*) at 3 somite (s) stage, as detected by WISH. Red and blue lines with double arrows indicate the width of the neural plate (np) in the anterior and posterior regions. All lines of the same color are equal in length. (I) The posterior body length in embryos in A–D, from three independent experiments (represented by different color squares), with the number of embryos indicated. (J) Average width of the neural plate in the anterior and posterior regions in embryos shown in E–H′ from two independent experiments (represented by different color squares), with the number of embryos indicated. Data are mean ± SEM. #, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; unpaired Student's t test. Scale bars, 200 µm.