Figure 8.
LIC1-CTD phosphorylation and Pin1 function are not required for interphase membrane transport.(A and B) Representative confocal images showing the localization of lysosomes (LAMP1, A), Golgi (COPI), and GalT (Golgi cargo) under the indicated conditions. NOC, nocodazole; Pin1i, Pin1 inhibitor. (C) Fraction of cells showing prominent perinuclear lysosomal localization under the indicated conditions. n = 3 experiments, 120 cells per experiment. (D and E) Immunoblots showing the expression of the indicated proteins; GalT is exogenously expressed in E. (F) Fraction of cells showing prominent perinuclear colocalization of COPI and GalT. n = 3 experiments, 75 cells per experiment. IB, immunoblot; dotted lines, cell boundaries. Scale bar = 30 µm. Error bars = mean ± SEM. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA).

LIC1-CTD phosphorylation and Pin1 function are not required for interphase membrane transport. (A and B) Representative confocal images showing the localization of lysosomes (LAMP1, A), Golgi (COPI), and GalT (Golgi cargo) under the indicated conditions. NOC, nocodazole; Pin1i, Pin1 inhibitor. (C) Fraction of cells showing prominent perinuclear lysosomal localization under the indicated conditions. n = 3 experiments, 120 cells per experiment. (D and E) Immunoblots showing the expression of the indicated proteins; GalT is exogenously expressed in E. (F) Fraction of cells showing prominent perinuclear colocalization of COPI and GalT. n = 3 experiments, 75 cells per experiment. IB, immunoblot; dotted lines, cell boundaries. Scale bar = 30 µm. Error bars = mean ± SEM. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA).

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