Anaphase spindle orientation, Dynein localization at the bud cortex and SPB, and the proportion of cells with plus end foci of Bik1. (a) Orientation of anaphase spindle in mother cell of kar9Δ cells with the indicated tubulin genotype. (b) Length of astral microtubules during anaphase. (c and d) Dyn1-3GFP fluorescence intensity at the bud cell cortex (c) and daughter-bound SPB (d) in the indicated cell types. (e) Percentage of cells with visible Bik1-3GFP foci at astral microtubule plus ends. Each panel shows mean ± SEM from three trials; in panel a, for Tub1 only, n = 20, 20, and 21; for WT-Tub1, n = 20, 23, and 23; for WT-Tub3, n = 20, 20, and 19; for Tub3 only, n = 20, 20, and 21. In panel b, plot shows microtubules observed from three trials with a total of 128, 124, 126, and 127 for Tub1 only, WT-Tub1, WT-Tub3, and Tub3 only, respectively. In panel c, for Tub1 only, n = 14, 17, and 16; for WT-Tub1, n = 12, 12, and 9; for WT-Tub3, n = 9, 11, and 11; for Tub3 only, n = 4, 15, and 17. In panel d, for Tub1 only, n = 34, 33, and 28; for WT-Tub1, n = 29, 30, and 33; for WT-Tub3, n = 42, 34, and 31; for Tub3 only, n = 30, 30, and 35. In panel e, for Tub1 only, n = 65, 62, and 63; for WT-Tub1, n = 73, 61, and 76; for WT-Tub3, n = 67, 63, and 70; for Tub3 only, n = 68, 63, and 81. *, P ≤ 0.05; **, P ≤ 0.01; n.s., not significant for Tub1 only versus Tub3 only (P = 0.94 in panel b, P = 0.479 in panel c, P = 0.721 in panel d, and P = 0.075 in panel e) using the unpaired, two-tailed Student’s t test. MT, microtubule.