Genetic interactions for TUB1 and TUB3. (a) Venn diagram of negative synthetic genetic interactions identified in our loss of TUB3 (blue; tub3Δ::TUB1) SGA screen and nonessential genes reported for simple TUB3 deletion (green; tub3Δ) SGA (Costanzo et al. 2016). Both screens identified 59 stringent interactions, with 8 hits common between both screens. (b) CellMap diagram showing negative synthetic genetic interaction networks with associated GO-term categories for the loss of TUB1 (yellow; tub1Δ::TUB3) or TUB3 (blue; tub3Δ::TUB1; Usaj et al., 2017). MVB, multivesicular body pathway (involved in ubiquitin-dependent sorting into endosomes and lysosome); RIM, regulator of IME2 pathway (involved in cell wall synthesis, meiosis, spore formation).