PINK1 contacts with CD63-positive endosomes. (a and b) MDA-MB-231 cells were transfected with GFP-tagged CD63 (GFP-CD63; green) and mitochondrial targeted DsRed (DsRed-mito; red) in combination with siRNAs targeting PINK1 (siPINK1) or nontargeting control (siNT). Transfected cells were incubated in the absence (DMSO) or presence (CCCP) of CCCP (10 µM) and imaged using a Zeiss LSM 880 Airyscan confocal microscope at 37°C with a frame rate of 0.59 s−1. Videos 1, 2, 3, and 4 were assembled from these images, and the presented images depict representative stills from the videos. Bar, 10 µm; bar in zoom, 1 µm. The number and duration of contacts between DsRed-mito– and GFP-CD63–positive structures were determined and are plotted as mean ± SEM; n = 3 independent experiments. *, P < 0.05; Mann-Whitney U test.
