Figure S4.
T-tubule dysmorphology in cavin4−/− zebrafish muscle is ameliorated by high expression of Bin1b. (A) General morphology of cavin4−/− zebrafish embryo at 72 hpf in comparison to a WT embryo. Scale bars: 500 µm. (B) Confocal image of a more severe EGFP-CaaX localization pattern in a transverse section of a 5-dpf cavin4−/− embryo. Asterisk indicates corresponding muscle fiber in higher magnification (shown as an inverted image) on right. Scale bars: 10 µm. (C) Confocal images of EGFP-CaaX in transverse sections of 10-dpf WT and cavin4−/− embryos. Asterisk indicates corresponding muscle fiber in higher magnification (shown as an inverted image) on right. Scale bars: 10 µm. (D) Confocal images of EGFP-CaaX in transverse sections of 30-dpf WT, cavin4+/−, and cavin4−/− embryos (bars: 20 µm). Asterisk indicates corresponding muscle fiber in higher magnification (shown as an inverted image) in the middle panel (bars: 10 µm). Images of muscle fibers in longitudinal orientation are shown as inverted images in far right panel; arrowheads indicate longitudinal tubules (scale bars: 10 µm). Cavin4+/− embryos are clutchmates of cavin4−/− embryos. (E) Confocal images of EGFP-CaaX in transverse sections of WT and cavin4−/− muscle fibers with transient overexpression of mKate2-Bin1b. Asterisks indicate corresponding muscle fibers; smaller panels on the far right (shown as inverted images) represent higher magnification of these fibers (top image: EGFP-CaaX; bottom image: mKate2-Bin1b). Scale bars: 10 µm.

T-tubule dysmorphology in cavin4−/− zebrafish muscle is ameliorated by high expression of Bin1b. (A) General morphology of cavin4−/− zebrafish embryo at 72 hpf in comparison to a WT embryo. Scale bars: 500 µm. (B) Confocal image of a more severe EGFP-CaaX localization pattern in a transverse section of a 5-dpf cavin4−/− embryo. Asterisk indicates corresponding muscle fiber in higher magnification (shown as an inverted image) on right. Scale bars: 10 µm. (C) Confocal images of EGFP-CaaX in transverse sections of 10-dpf WT and cavin4−/− embryos. Asterisk indicates corresponding muscle fiber in higher magnification (shown as an inverted image) on right. Scale bars: 10 µm. (D) Confocal images of EGFP-CaaX in transverse sections of 30-dpf WT, cavin4+/−, and cavin4−/− embryos (bars: 20 µm). Asterisk indicates corresponding muscle fiber in higher magnification (shown as an inverted image) in the middle panel (bars: 10 µm). Images of muscle fibers in longitudinal orientation are shown as inverted images in far right panel; arrowheads indicate longitudinal tubules (scale bars: 10 µm). Cavin4+/− embryos are clutchmates of cavin4−/− embryos. (E) Confocal images of EGFP-CaaX in transverse sections of WT and cavin4−/− muscle fibers with transient overexpression of mKate2-Bin1b. Asterisks indicate corresponding muscle fibers; smaller panels on the far right (shown as inverted images) represent higher magnification of these fibers (top image: EGFP-CaaX; bottom image: mKate2-Bin1b). Scale bars: 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal