Identifying factors that are involved in Cnm1-induced contact sites. (A) A schematic representation of the systematic screen to find modulators of Cnm1 overexpression. Cnm1, overexpressed under the strong TEF2 promoter, and the nuclear envelope protein Nsg1, tagged with GFP on its C terminus, were integrated into the deletion/hypomorphic allele library. In this library, each colony harbors a loss-of-function mutant in each of the ∼6,000 yeast genes. Prior to imaging, cells were stained with a red mitochondrial dye (MitoTracker Orange). The genes that when mutated resulted in partial or reduced mitochondrial clustering around the nucleus were considered as hits. Representative images of the mutants labeled in white are shown. Scale bar, 5 µm. (B) A table of all deleted genes that caused reduced mitochondrial clustering on the background of Cnm1 overexpression arranged by alphabetical order. GPI, glycosylphosphatidylinositol. Protein localization and description are presented in the middle and right columns, respectively. For a full list of the mutant genes that resulted in partial clustering, see Table S2.