Frequency of micronuclei is increased in thymic lymphoma tissues from Kif18a mutants. (A) Representative images of nuclear envelopes for primary nuclei and micronuclei occurring in healthy mouse tissue (top) and in thymic lymphoma (middle and bottom) from the indicated genotypes. Sections were stained with Hoechst (DNA, blue), ezrin (plasma membrane, red), and lamin A/C (nuclear envelope, green). Arrowheads indicate micronuclei; yellow arrowheads show micronuclei with complete lamin A/C, and red arrowheads show incomplete micronuclei. (B) Plot showing the percentage of micronucleated cells in thymic lymphoma tissues for indicated genotypes. Data points represent individual biological samples. n = 3 biological replicates per genotype: Kif18a+/+, Trp53tm1 Tyj/tm1Tyj (n = 1,632 cells); Kif18agcd2/gcd2, Trp53tm1 Tyj/tm1Tyj (n = 1,615 cells). *, P < 0.01 (Table S4). Statistical comparison was made using χ2 analysis. (C) Plot showing the percentage of micronuclei lacking recruitment of lamin A/C in thymic lymphoma tissues for indicated genotypes. Data points represent individual biological samples. n = 3 biological replicates per genotype: Kif18a+/+, Trp53tm1 Tyj/tm1Tyj (n = 54 micronuclei); Kif18agcd2/gcd2, Trp53tm1 Tyj/tm1Tyj (n = 90 micronuclei). P = 0.29, ns (Table S5). Statistical comparison was made using χ2 analysis.
