Primary cells extracted from murine thymic lymphoma exhibit high levels of aneuploidy. (A) Representative images of metaphase chromosome spreads prepared from individual biological replicates of primary cells extracted from murine thymic lymphoma from Kif18a+/+, Trp53tm1 Tyj/tm1Tyj and Kif18agcd2/gcd2, Trp53tm1 Tyj/tm1Tyj mice. Indicated chromosome count number displayed in lower right corners. (B) Plot showing the chromosome copy number observed in primary lymphoma cells from individual biological replicates (colors indicate data from individual biological replicates). n = 3 biological samples from Kif18a+/+, Trp53tm1 Tyj/tm1Tyj mice (sample 1, 18 cells; sample 2, 20 cells; sample 3, 23 cells); n = 2 biological samples from Kif18agcd2/gcd2, Trp53tm1 Tyj/tm1Tyj mice (sample 1, 21 cells; sample 2, 10 cells). Dashed lines indicate chromosome copy number for euploidy (40 chromosomes) and tetraploidy (80 chromosomes).
