Figure S4.
Representative fields of view and quantification of cells with cleaved IETD substrate in HCT116 cells treated with or without TRAIL in the absence or presence of Y-27632 or caspase inhibitors.(A) Representative images and quantification of cells with cleaved IETD substrate in HCT116 cells treated with or without TRAIL in the absence or presence of z-VAD-fmk, z-IETD-fmk, or Y-27632. Scale bar: 25 μm. Data are shown as individual values for each experiment as well as mean ± SEM from three experiments. ****, P < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparison test. (B) Representative images of Hoechst and LysoTracker staining in HCT116 cells treated with or without TRAIL in the absence or presence of z-VAD-fmk or Y-27632. Arrows indicate entosis events. Scale bar: 25 μm. (C) Representative images of Hoechst-stained (blue) HCT116 CASP8−/− cells (pX330-Cas9-sgCASP8; green). Quantification of entotic events in control, TRAIL-treated, or glucose-starved HCT116 WT and CASP8−/− cells. More than 500 cells were manually quantified from two experiments. Scale bar: 20 μm. Data are shown as mean ± SD. *, P < 0.05 by one-way ANOVA followed by Tukey’s multiple comparison test.

Representative fields of view and quantification of cells with cleaved IETD substrate in HCT116 cells treated with or without TRAIL in the absence or presence of Y-27632 or caspase inhibitors. (A) Representative images and quantification of cells with cleaved IETD substrate in HCT116 cells treated with or without TRAIL in the absence or presence of z-VAD-fmk, z-IETD-fmk, or Y-27632. Scale bar: 25 μm. Data are shown as individual values for each experiment as well as mean ± SEM from three experiments. ****, P < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparison test. (B) Representative images of Hoechst and LysoTracker staining in HCT116 cells treated with or without TRAIL in the absence or presence of z-VAD-fmk or Y-27632. Arrows indicate entosis events. Scale bar: 25 μm. (C) Representative images of Hoechst-stained (blue) HCT116 CASP8−/− cells (pX330-Cas9-sgCASP8; green). Quantification of entotic events in control, TRAIL-treated, or glucose-starved HCT116 WT and CASP8−/− cells. More than 500 cells were manually quantified from two experiments. Scale bar: 20 μm. Data are shown as mean ± SD. *, P < 0.05 by one-way ANOVA followed by Tukey’s multiple comparison test.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal