Ultrastructural localization of LC3 in negative control and middle-stage inner cell shown in Fig. 3 . (A) Representative TEM images showing ultrastructural localization of LC3 in negative control and middle-stage inner cell shown in Fig. 3 D. Yellow circles indicate gold particles. Quantification of gold particles in early-, middle-, and late-stage inner and outer cells shown in Fig. 3. Statistical significance was tested using one-way ANOVA followed by Tukey’s multiple comparison test. (B) Quantification of LAMP1 and LysoTracker mean fluorescence intensity (MFI) in depicted inner cell undergoing entotic cell death shown in Fig. 3 F. (C) Quantification of cathepsin B activity in early- and late-stage entotic cells corresponding to Fig. 3 G. n = 37 (early stage) and n = 28 (late stage) inner cells were quantified from three experiments. Data are shown as individual values for each cell as well as median and quartiles. ****, P < 0.0001 by unpaired two-tailed t test. IC, inner cell; OC, outer cell.