Shape of the fusion pore. (A–C) Original noncolored virtual z-slices through tomograms shown in Fig. 3, C and D. (D) Four virtual z-slices through a tomogram showing two large pores with irregular edges. These two pores are likely joined in a single pore outside the reconstructed tomographic volume. On the top line, the CW is colored in transparent purple to help visualization. Corresponding models of the outer leaflet of the PM are on the right. The position of each z-slice is indicated by the purple lines on the model, where the z-slice intersects the green PM outline at perpendicular angle. Z-slices are ordered from bottom (left edge of the model image) to top (right edge; see Video 3, Video 4, Video 5, and Video 6). Scale bars, 100 nm.