Centrosome and granule docking. Related to Fig. 4, g–l. GzmB-TdTomato OTI CTLs expressing Lifeact-EGFP and BFP-PACT interacting with EL4, pulsed with N4, T4, or G4 peptides, were segmented using Imaris to measure relative distances to the synapse. R was used to filter granules for concomitant centrosome docking and granule delivery (<0.5 µm of the synapse). Red spots show granules reaching the synapse on blue lines showing centrosome distance from the synapse, from total CTL of N4 = 10, T4 = 9, and G4 = 10. Representatives were taken from these for Fig. 4, g–l.