Representative time-lapse images show the composition of released centromeres. (A) The total intensity of splitting centromere fragments a and b is equal to intact centromere c (green: MajSAT-mClover; gray: H2B-mCherry). Time from NEBD. Data from nine time points. P value from paired t test, P = 0.875. Scale bar: 2 µm. (B) The released centromere parts are marked by arrows (blue: Hoechst; green: MajSAT-mClover; red: H2B-mCherry). Scale bar: 5 µm (yellow) and 2 µm (white).