Cen-RNA depletion induced meiotic defects. (A–C) Inter-kinetochore distance (A), alignment distance (B), and bivalent angle (C) in bivalents from Cen-ASO– or 5MM-ASO–injected oocytes (200 bivalents from 10 oocytes) at 6 h after NEBD (****, P < 0.0001, t test). Schematic shows the measurement being made. Error bars indicate SD. (D) Representative time-lapse image of a Cen-ASO–injected oocyte expressing H2B-mCherry (gray) and MajSAT-mClover (green). A nonaligned bivalent is labeled by blue arrowheads. Times are relative to anaphase. Scale bar: 5 µm. (E) Percentage of oocytes with nonaligned bivalents following injection with either Cen-ASO or 5MM-ASO (****, P < 0.0001, χ2 test). (F) Representative time-lapse image of a Cen-ASO–injected oocyte expressing H2B-mCherry (gray) and MajSAT-mClover (green). A lagging chromosome is labeled by the red arrowheads. Times are relative to anaphase. Scale bar: 5 µm. (G) Lagging chromosome rates in oocytes injected with either Cen-ASO or 5MM-ASO (****, P < 0.0001, χ2 test). The experiments were performed twice.
