Localized sterol transfer is required for endocytic uptake only in mother cells. (A–D) Average ± SEM life span and t test P values of Sla1-GFP (A, B, and D) or Sla2-GFP (C) cortical patches in mother and daughter cells of WT cells or the indicated mutants, untreated, mock treated (DMSO, +), or treated with filipin (D). Significant differences are shown in red (n > 150). Kymographs of time-lapse fluorescence movies of cortical Sla1-GFP patches for each experimental condition are shown. Images were taken every 0.5 s.