Figure 4.
Asymmetric regulation of sterol-dependent endocytosis in yeast.(A) Average ± SEM life span and t test P values of Sla1-GFP cortical patches in mother and daughter cells of WT cells mock treated (DMSO, +) or treated with the indicated drugs, concentrations, and times. Significant differences are shown in red (n > 150). Kymographs of time-lapse fluorescence movies of cortical Sla1-GFP patches for each experimental condition shown. Images were taken every 0.5 s. (B) Merged kymographs of cortical patches (upper panels) of time-lapse fluorescence movies of cells expressing Sla1-GFP and Abp1-mCherry, mock treated (DMSO) or treated 5 min with FPM or OSW-1. Images taken every 0.5 s. Average ± SEM of cortical Abp1-mCherry patch RFI in mock-treated cells (blue) or under the indicated treatments (red; n > 60).

Asymmetric regulation of sterol-dependent endocytosis in yeast. (A) Average ± SEM life span and t test P values of Sla1-GFP cortical patches in mother and daughter cells of WT cells mock treated (DMSO, +) or treated with the indicated drugs, concentrations, and times. Significant differences are shown in red (n > 150). Kymographs of time-lapse fluorescence movies of cortical Sla1-GFP patches for each experimental condition shown. Images were taken every 0.5 s. (B) Merged kymographs of cortical patches (upper panels) of time-lapse fluorescence movies of cells expressing Sla1-GFP and Abp1-mCherry, mock treated (DMSO) or treated 5 min with FPM or OSW-1. Images taken every 0.5 s. Average ± SEM of cortical Abp1-mCherry patch RFI in mock-treated cells (blue) or under the indicated treatments (red; n > 60).

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