Figure S1.
Asymmetric distribution of GFP-D4H labeling in yeast.(A) Scheme of the GFP-D4H probe that recognizes sterols. (B) Kymographs of time-lapse fluorescence movies of Sla1-mCherry cortical patches of WT cells expressing or not GFP-D4H. Images were taken every 1 s. A graph of average ± SEM life span of Sla1-mCherry cortical patches with Student’st test P values is shown (n > 150). (C) Ergosterol late biosynthetic pathway. Ergs localized at ERSESs are shown in blue. Steps inhibited by TBF and FPM are indicated. (D–G) DIC and epifluorescence micrographs of the indicated strains expressing GFP-D4H, grown at 25°C or upon shift at 37°C for 1 h (F). Arrowheads point to vesicle-like structures. Graphs in F and G represent average ± SEM frequencies of cells showing polarized staining. n > 100 cells in three experiments.

Asymmetric distribution of GFP-D4H labeling in yeast. (A) Scheme of the GFP-D4H probe that recognizes sterols. (B) Kymographs of time-lapse fluorescence movies of Sla1-mCherry cortical patches of WT cells expressing or not GFP-D4H. Images were taken every 1 s. A graph of average ± SEM life span of Sla1-mCherry cortical patches with Student’st test P values is shown (n > 150). (C) Ergosterol late biosynthetic pathway. Ergs localized at ERSESs are shown in blue. Steps inhibited by TBF and FPM are indicated. (D–G) DIC and epifluorescence micrographs of the indicated strains expressing GFP-D4H, grown at 25°C or upon shift at 37°C for 1 h (F). Arrowheads point to vesicle-like structures. Graphs in F and G represent average ± SEM frequencies of cells showing polarized staining. n > 100 cells in three experiments.

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