Effect of TPD54 depletion on the shape of RPE1 cells. Cell shape descriptors for control (gray) or TPD54-depleted (blue) RPE1 cells on fibronectin-coated dishes. Valid shapes were analyzed from n_cell = 288 (siCtrl) and n_cell = 151 (siTPD54). (A–K) Violin plots where dots represent individual cells and marker indicates the mean, showing minor axis length (A); major axis length (B); cell perimeter (C); cell area (D); aspect ratio, minor/major axes (E); symmetry, ratio of the cell area to the area of the cell footprint reflected on its major axis (F); convex area, area of a convex hull enclosing the cell perimeter (G); solidity, ratio of cell area to convex area (H); extent, ratio of cell area to the area of a bounding box (I); maximum distance to the perimeter from the cell center (J); and minimum distance to the perimeter from the cell center (K). (L) A plot of minor axis length as a function of major axis length. Each cell in the dataset is represented by a bubble, the size of which corresponds to cell area.