TPD54-depleted cells make larger contacts with their substrate. (A) Example micrographs of control and TPD54-depleted RPE1 cells migrating on fibronectin. The perimeter of the cell is outlined (white). Scale bar, 10 µm. (B) Superplot showing the area of control versus TPD54-depleted RPE1 cells migrating on fibronectin. Dots represent individual cells, colors represent different experiments, and the population mean is outlined in black. P value from Student’s test. n_cell = 288,151; n_exp = 4. More statistics of cell shape are shown in Fig. S5.