Effect of TPD52 or TPD53 depletion on migration of RPE1 cells. Statistics of control (gray), TPD52-depleted (green), or TPD53-depleted (purple) RPE1 cells migrating on fibronectin-coated dishes. Data from Fig. 8. (A and H) Cumulative distance over time (mean ± SEM). (B and I) Instantaneous speed over time (mean ± SEM). (C and J) Histogram of instantaneous speed. (D and K) Histogram of cell turning. The angle distribution for all tracks in the analysis measured from one trajectory to the next. (E and L) Violin plot of the fastest time taken for each cell to traverse a segment of 25 µm in a track. Dots represent individual cells, and marker indicates the mean. (F and M) Mean squared displacement (mean ± SEM). (G and N) Direction autocorrelation (mean ± SEM). (O) Western blot to show depletion of TPD53 under the conditions of the migration experiment. Depletion of TPD52 was not possible to assess due to lack of specific antibodies.