Effect of TPD54 depletion on migration of RPE1 cells. Statistics of control (gray) or TPD54-depleted (blue) RPE1 cells migrating on fibronectin-coated dishes. Data from Fig. 8. (A) Cumulative distance over time (mean ± SEM). (B) Instantaneous speed over time (mean ± SEM). (C) Histogram of instantaneous speed. (D) Histogram of cell turning. The angle distribution for all tracks in the analysis measured from one trajectory to the next. (E) Violin plot of the fastest time taken for each cell to traverse a segment of 25 µm in a track. Dots represent individual cells, marker indicates the mean. (F) Mean squared displacement (mean ± SEM). (G) Direction autocorrelation (mean ± SEM). (H) Overlay of all tracks in the dataset shown as a heatmap (left), bootstrapped and rotated view of cell tracks to visualize the average explored space by cells in the dataset. Density is shown by the log color scale indicated. (I) Image quilt of a sample of cell tracks from the dataset. Each track is shown in its original orientation, arrayed on a grid from the shortest distance traveled to the longest. (J) Sparkline image of a diagonal sample through the image quilt (I). To visualize directionality, tracks are rotated such that the end point of the track due east from the start.