Migration of RPE1 cells on laminin. Control (gray) or TPD54-depleted (blue) RPE1 cells migrating on laminin-coated dishes. n cell = 286–297, nexp = 2. (A) Cumulative distance over time (mean ± SEM). (B) Instantaneous speed over time (mean ± SEM). (C) Histogram of instantaneous speed. (D) Directionality ratio over time (mean ± SEM). (E) Mean squared displacement (MSD; mean ± SEM). (F) Direction autocorrelation (mean ± SEM). (G) Histogram of cell turning. The angle distribution for all tracks in the analysis measured from one trajectory to the next. (H) Violin plot of average speed of each cell. (I) Violin plot of the fastest time taken for each cell to traverse a “segment” of 25 µm in a track. Dots represent individual cells, and marker indicates the mean.
