Figure 7.

Spatiotemporal variance of GFP-Rab proteins. (A) Heatmap showing the Rab screen data for TPD52, TPD53 (from Fig. 6, A and C), and TPD54 (Larocque et al., 2020). Z-score of the values is color-coded and depicted as a purple line. Distribution of Z-values is shown in the color key. (B) Euler plot to show the Rabs identified in the screens and how they are linked to TPD52-like proteins. (C) Scatter dot plot of variance of fluorescence for the indicated GFP-Rab proteins expressed in HeLa cells. Rabs identified as present on INVs by hierarchical clustering of vesicle capture screens are indicated by the colors indicated in B. Dots represent individual cells, and bars indicates the mean ± SD. The mean ± SD for GFP (control) is also shown as a black line and gray zone, across the plot.

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