Figure 6.
Screening Rab GTPases that are associated with TPD52 and TPD53 INVs.(A and C) Quantification of the change in mitochondrial fluorescence intensity of GFP or GFP-Rabs 2 min after rerouting of mCherry-FKBP-TPD52 (A) or mCherry-FKBP-TPD53 (C) to dark MitoTrap with 200 nM rapamycin. Fpost/Fpre is the average fluorescence of indicated GFP-Rab at mitochondria after rerouting (“post”) divided by the fluorescence in the same region before (“pre”). Dots represent values for individual cells across three independent trials. Black bars represent mean ± SD. The mean ± SD for GFP (control) is also shown as a black line and gray zone, down the plot. Red indicates P < 0.05; Dunnett’s post hoc test, GFP as control. (B and D) Effect size and bootstrap 95% confidence interval of the data in A and C, respectively.

Screening Rab GTPases that are associated with TPD52 and TPD53 INVs. (A and C) Quantification of the change in mitochondrial fluorescence intensity of GFP or GFP-Rabs 2 min after rerouting of mCherry-FKBP-TPD52 (A) or mCherry-FKBP-TPD53 (C) to dark MitoTrap with 200 nM rapamycin. Fpost/Fpre is the average fluorescence of indicated GFP-Rab at mitochondria after rerouting (“post”) divided by the fluorescence in the same region before (“pre”). Dots represent values for individual cells across three independent trials. Black bars represent mean ± SD. The mean ± SD for GFP (control) is also shown as a black line and gray zone, down the plot. Red indicates P < 0.05; Dunnett’s post hoc test, GFP as control. (B and D) Effect size and bootstrap 95% confidence interval of the data in A and C, respectively.

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