Localization of the constructs comprising the β-propeller region of VPS13D only. (A) Cartoon depicting full-length VPS13D and the VPS13D fragments used in the imaging experiment shown in B and C. (B and C) Confocal images of representative examples of the heterogeneous localization of two mCherry-tagged VPS13D fragments in COS7 cells coexpressing EGFP-Miro1. The first column shows a cytosolic localization; the second column shows the presence of small aggregates; and the third and fourth columns show the colocalization of VPS13D with Miro on mitochondria whose localization is disrupted. Scale bars, 10 µm. (D) List of VPS13D fragments containing the β-propeller region that were tested for Miro-induced recruitment to mitochondria. All of them behaved similarly. The constructs used for fields in B and C are highlighted in green.