A transcript variant of Miro1 preferentially targeted to peroxisomes recruits VPS13D to peroxisomes. (A) Domain organization of two splice variants of Miro1 (Okumoto et al., 2018). TM, transmembrane. In the alternative splice variant 4 (Miro1v4), two extra exons are included that encode 73 additional amino acids in the C-terminal region of Miro1. These amino acids promote the interaction of the protein with the chaperone Pex19, which leads to the predominant insertion of this variant into the peroxisomal membrane, although a pool of this variant still localizes to mitochondria. (B) COS7 cell showing the recruitment of VPS13D^Halo to peroxisomes (visualized by the peroxisomal luminal marker mScarlet-SRL) upon coexpression with transcript variant Miro1v4. The insets show colocalization with the peroxisomal marker (green arrowheads) and a weaker signal for both VPS13D and Miro1v4 on mitochondria (white arrowheads). Scale bars, 10 µm in the main panel and 3 µm in the insets.