In support of Fig. 3: Spatial analysis of de novo centriole biogenesis in fly explants at different Plk4 concentrations. (A) De novo centriole biogenesis in fly explants at high Plk4 concentration. Left: 2D z projections of the positions of centrioles at the moment they were first detected in the explants, 254 centrioles measured in 68 explants (Observations) and 272 centrioles from 68 in silico explants (Simulations). All coordinates were normalized to the measured explant diameter. Right: Distributions of observed and simulated inter-event distances measured in 3D for the first four centrosomes formed de novo in the explants. (B) De novo centriole biogenesis at lower Plk4 concentration. Left: z projections of the positions of centrioles at the moment they were first detected in the explants, 75 centrioles measured in 20 explants (Observations) and 80 centrioles from 20 in silico explants (Simulations). All coordinates were normalized to the measured explant diameter. Right: Distributions of observed and simulated inter-event distances measured in 3D for the first four centrosomes formed de novo in the explants, at the lowest Plk4 overexpression (0.16 relative concentration of Plk4). The gray envelope indicates the 95% confidence interval (from quantile 0.025 to 0.975) for the simulated data.