Figure 8.
LLPS is required for the proper recruitment of HR- and NHEJ-related proteins.(A) HeLa WT cells were transiently transfected with KU80-GFP–expressing plasmids and then incubated with 2% 1,6-HD for 30 min before laser microirradiation. Upper panel: Representative micrographs of selected time points. Lower panel: Time course of KU80 recruitment. (B) HeLa WT cells were transiently transfected with NBS1-GFP–expressing plasmids and then incubated with 2% 1,6-HD for 30 min before laser microirradiation. Upper panel: Representative micrographs of selected time points. Lower panel: Time course of NBS1 recruitment. (C) Recruitment of KU80-GFP in FUS-KO cells either untransfected or transfected with FUS WT, FUS RK, or FUS YS. Upper panel: Representative micrographs of selected time points. Lower panel: Time course of KU80 recruitment. In all graphs, data are plotted as normalized average ± SEM. Scale bars: 2 µm. Arrows indicate microirradiated area.

LLPS is required for the proper recruitment of HR- and NHEJ-related proteins. (A) HeLa WT cells were transiently transfected with KU80-GFP–expressing plasmids and then incubated with 2% 1,6-HD for 30 min before laser microirradiation. Upper panel: Representative micrographs of selected time points. Lower panel: Time course of KU80 recruitment. (B) HeLa WT cells were transiently transfected with NBS1-GFP–expressing plasmids and then incubated with 2% 1,6-HD for 30 min before laser microirradiation. Upper panel: Representative micrographs of selected time points. Lower panel: Time course of NBS1 recruitment. (C) Recruitment of KU80-GFP in FUS-KO cells either untransfected or transfected with FUS WT, FUS RK, or FUS YS. Upper panel: Representative micrographs of selected time points. Lower panel: Time course of KU80 recruitment. In all graphs, data are plotted as normalized average ± SEM. Scale bars: 2 µm. Arrows indicate microirradiated area.

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