Loss of FUS specifically affects SFPQ but not XRCC1 recruitment to DSB. (A) WT HeLa cells were transiently cotransfected with SFPQ-GFP and XRCC1-RFP plasmids and submitted to laser microirradiation as described in Materials and methods. Recruitment of these proteins was assessed for a 3-min period, and images were taken every 20 s. Scale bar: 2 µm. (B) Recruitment and accumulation of SFPQ, as shown in Fig. 4 D, is severely impaired in FUS-KO cells. (C) Recruitment of XRCC1 is very high (saturated fluorescence signal) and the same for WT and FUS-KO cells. Error bars represent SE.