Microirradiation experiments are not influenced by differential expression of KU80-GFP or NBS1-GFP in WT versus FUS-KO cell lines. (A) KU80-GFP is recruited to the microirradiated area and colocalizes with γH2AX staining. Scale bar: 2 µm. (B) The raw fluorescence intensity of HeLa WT and FUS-KO cells transiently transfected with KU80-GFP was assessed to rule out the possibility that differential expression levels could affect the observed effect in Fig. 3 A. (C) The raw fluorescence intensity of HeLa WT and FUS-KO cells transiently transfected with NBS1-GFP was assessed to rule out the possibility that differential expression levels could affect the observed effect in Fig. 3 B.