Figure 2.
Loss of FUS perturbs DDR signaling and foci formation upon genotoxic insult.(A) Representative Western blot of DDR proteins in HeLa WT and FUS-KO cells upon ETO treatment. Cells were treated with 10 µM ETO for 1 h and were allowed to recover in ETO-free medium for 2 h (ETO release). Cells were collected at the indicated time points, lysed in the presence of phosphatase inhibitors, separated on a gradient SDS-PAGE, and processed for Western blotting (loading control: Actin). MW, molecular weight. (B) Quantification of the blots from two independent experiments as in A (n = 2). (C) Quantification of ETO-induced γH2AX foci. Data are from two biological replicates (170 cells each). Statistics: two-way ANOVA, Bonferroni post hoc test. (D) Quantification of ETO-induced 53BP1 foci analyzed as in C. *, P < 0.05; ***, P < 0.001.

Loss of FUS perturbs DDR signaling and foci formation upon genotoxic insult. (A) Representative Western blot of DDR proteins in HeLa WT and FUS-KO cells upon ETO treatment. Cells were treated with 10 µM ETO for 1 h and were allowed to recover in ETO-free medium for 2 h (ETO release). Cells were collected at the indicated time points, lysed in the presence of phosphatase inhibitors, separated on a gradient SDS-PAGE, and processed for Western blotting (loading control: Actin). MW, molecular weight. (B) Quantification of the blots from two independent experiments as in A (n = 2). (C) Quantification of ETO-induced γH2AX foci. Data are from two biological replicates (170 cells each). Statistics: two-way ANOVA, Bonferroni post hoc test. (D) Quantification of ETO-induced 53BP1 foci analyzed as in C. *, P < 0.05; ***, P < 0.001.

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