Loss of FUS does not affect SG assembly. HeLa WT and FUS-KO cells were stressed by treatment with 0.5 mM sodium arsenite for 1 h and immunostained for FUS and TIA-1 (a eukaryotic SG marker). Consistent with previous observations (Sama et al., 2013; Hock et al., 2018), FUS remained nuclear in WT cells upon arsenite incubation, and SGs could be detected in the cytoplasm of both WT and FUS-KO cells. Scale bar: 50 µm (10 µm for cropped image).