Growth factor activation at FAs. (A) HeLa cells FCS starved for 18 h (−FCS) or starved and stimulated with EGF or IGF for 10 min were analyzed by immunofluorescence (left) and immunoblotting (right). (B) Cells were starved and stimulated with IGF, EGF, or amino acids. FAs remaining on coverslips after removal of cell bodies were immunostained using paxillin and p-IGFR1, p-EGFR, or SLC3A2 antibodies. (C) Cells were starved and stimulated as indicated and immunostained for p-EGFR, p-IGFR1, SLC3A2, and LAMP1, and colocalization at the cell periphery (Manders coefficient) was quantified. (D and E) Interaction network analysis of growth factor receptors and mTOR-associated proteins (D) and lysosome-associated proteins (E) detected in adhesion complex proteomes (meta-adhesome datasets). Proteins (nodes) are annotated with gene names for clarity. Error bars represent SEM; n = 3 independent experiments. Scale bars, 20 µm (insets, 10 µm). Nuclei were visualized with DAPI; original localization of nuclei in FA preparations (B) is indicated by dashed circles. For C, ***, P < 0.001; two-sided Student’s t test.