Figure S5.
The focused distribution of the polarisome is actin dependent. (A) Images of myo2-66 cells from Fig. 4 D at 27°C categorized as displaying a diffuse (Spa2-mCherry at bud neck, in misplaced clusters, or scattered), a cortically spread, or a focused distribution of Spa2-mCherry. (B) As in Figs. 4 E and 6 F but showing the representative images of cells expressing GFP-Pea2. (C) The distribution of Myo2 is altered in bni1Δ and msb3Δ msb4Δ cells. Left: Fluorescence microscopy of WT, bni1Δ, or msb3Δmsb4Δ cells expressing Myo2-GFP. Right: Quantification of cells from the left panel displaying focused, cortically spread, or scattered Myo2-GFP distributions (nWT = 237, nbni1Δ = 239, nmsb3Δ msb3Δ = 228). All scale bars: 5 µm.

The focused distribution of the polarisome is actin dependent. (A) Images of myo2-66 cells from Fig. 4 D at 27°C categorized as displaying a diffuse (Spa2-mCherry at bud neck, in misplaced clusters, or scattered), a cortically spread, or a focused distribution of Spa2-mCherry. (B) As in Figs. 4 E and 6 F but showing the representative images of cells expressing GFP-Pea2. (C) The distribution of Myo2 is altered in bni1Δ and msb3Δ msb4Δ cells. Left: Fluorescence microscopy of WT, bni1Δ, or msb3Δmsb4Δ cells expressing Myo2-GFP. Right: Quantification of cells from the left panel displaying focused, cortically spread, or scattered Myo2-GFP distributions (nWT = 237, nbni1Δ = 239, nmsb3Δ msb3Δ = 228). All scale bars: 5 µm.

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