Figure S1.
Screening for interaction partners of Myo2. (A) Split-Ub interaction assay of 548 yeast strains coexpressing PMET17-Myo2CRU (left) or PMET17-CBDCRU (right) each with a different Nub fusion protein. In each case, cells of four independent matings were spotted as quadruplets on SD medium containing 5-FOA, 150 µM copper sulfate, and 70 µM methionine. Shown is the growth of the diploid yeast cells after 3 d at 30°C. Growth indicates protein–protein interaction. (B) Matrix revealing the identities of the respective Nub-fusions in A.

Screening for interaction partners of Myo2. (A) Split-Ub interaction assay of 548 yeast strains coexpressing PMET17-Myo2CRU (left) or PMET17-CBDCRU (right) each with a different Nub fusion protein. In each case, cells of four independent matings were spotted as quadruplets on SD medium containing 5-FOA, 150 µM copper sulfate, and 70 µM methionine. Shown is the growth of the diploid yeast cells after 3 d at 30°C. Growth indicates protein–protein interaction. (B) Matrix revealing the identities of the respective Nub-fusions in A.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal