Figure S1.
Depletion of PCM components in two-, one-, and zero-centrosome cells. (A–C) Western blot analysis of the efficiency of protein depletion of CEP192 (A), CDK5RAP2 (B), and pericentrin (C) after 48 h of siRNA transfection in HeLa cells. (D–F) Quantification of depleted centrosomal CEP192 (D), CDK5RAP2 (E), and pericentrin (F). Line and error bars represent the mean and SD. The Mann–Whitney U test (two tailed) was used to obtain a P value. *, P < 0.0001. (G–I) Time-lapse observation of the structure of microtubules upon siRNA treatment against the indicated proteins. DMSO-treated two-centrosome (G), centrinone-treated one-centrosome (H) and zero-centrosome (I) HeLa cells expressing EGFP-centrin1 and mCherry-NuMA were observed with a 40× objective. Green and gray represent EGFP-centrin1 and SiR-tubulin, respectively. mCherry-NuMA is not shown. Z-projections: 10 planes, 2.2 µm apart. Scale bar, 10 µm. Time zero corresponds to NEBD.

Depletion of PCM components in two-, one-, and zero-centrosome cells. (A–C) Western blot analysis of the efficiency of protein depletion of CEP192 (A), CDK5RAP2 (B), and pericentrin (C) after 48 h of siRNA transfection in HeLa cells. (D–F) Quantification of depleted centrosomal CEP192 (D), CDK5RAP2 (E), and pericentrin (F). Line and error bars represent the mean and SD. The Mann–Whitney U test (two tailed) was used to obtain a P value. *, P < 0.0001. (G–I) Time-lapse observation of the structure of microtubules upon siRNA treatment against the indicated proteins. DMSO-treated two-centrosome (G), centrinone-treated one-centrosome (H) and zero-centrosome (I) HeLa cells expressing EGFP-centrin1 and mCherry-NuMA were observed with a 40× objective. Green and gray represent EGFP-centrin1 and SiR-tubulin, respectively. mCherry-NuMA is not shown. Z-projections: 10 planes, 2.2 µm apart. Scale bar, 10 µm. Time zero corresponds to NEBD.

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