Figure 6.
Functions of kat-60L1 in leg receptors. (A) Representative confocal images (lateral view) showing the membrane, NompC, and tubulin labeling of the outer segment. Upper left: dcx-emap-gal4/uas-cd4-tdTom. Upper middle: NompC-gfp-KI. Upper right: dcx-emap-gal4/uas-gfp-αtub84B. Lower left: dcx-emap-gal4/uas-cd4-tdTom; c01236/BE6. Lower middle: NompC-gfp-KI; c01236/BE6. Lower right: dcx-emap-gal4/uas-gfp-αtub84B; c01236/BE6. OS, outer segment; d, dendritic inner segment. Scale bar, 5 µm. (B–D) Statistical quantification of the lateral area of the outer segments (B), NompC-GFP signal in the MO (C), and GFP-tubulin signal in the outer and inner segments (D). (E) Representative ET slice images (lateral view) of the outer segment in wild type (left) and c01236/BE6 (right). Note that in mutant cells, several microtubules (red arrowheads), including a doublet (yellow arrowheads), spanned across the TB and MO. Scale bars, 0.5 µm. (F) Representative ET slice images (cross-sectional view) of the outer segment in c01236/BE6. Note that the number of microtubules in the outer segment was largely reduced. Scale bar, 0.5 µm. (G) Statistical quantification of the ratio between the number of microtubules that spanned across the TB and MO (see Materials and methods) to the number of MO microtubules. (H) Representative confocal images showing Patronin-RFP signals in the leg receptors of wild type (patronin-rfp-KI) and c01236/BE6 (patronin-rfp-KI; c01236/BE6). White arrowhead, the MO. Yellow arrowhead, the proximal end of the TB. Green arrowhead, dendritic inner segment. Scale bar, 5 µm. (I) Statistical quantification of Patronin-RFP signal at the distal region of the inner segment in wild type and c01236/BE6. (J) Representative confocal snapshots of EB1 comets in wild type (left, EB1-tdgfp-KI) and c01236/BE6 (right, EB1-tdgfp-KI; c01236/BE6). Scale bar, 5 µm. Also see Video 2. (K) Statistical quantification of EB1 comet frequency in the inner segment. In B–D, G, I, and K, data are presented as mean ± SD with scattered data points. Two-sided unpaired Student’s t test was used for statistical analysis. *, P < 0.05; ***, P < 0.001. The number of cells is indicated.

Functions of kat-60L1 in leg receptors. (A) Representative confocal images (lateral view) showing the membrane, NompC, and tubulin labeling of the outer segment. Upper left: dcx-emap-gal4/uas-cd4-tdTom. Upper middle: NompC-gfp-KI. Upper right: dcx-emap-gal4/uas-gfp-αtub84B. Lower left: dcx-emap-gal4/uas-cd4-tdTom; c01236/BE6. Lower middle: NompC-gfp-KI; c01236/BE6. Lower right: dcx-emap-gal4/uas-gfp-αtub84B; c01236/BE6. OS, outer segment; d, dendritic inner segment. Scale bar, 5 µm. (B–D) Statistical quantification of the lateral area of the outer segments (B), NompC-GFP signal in the MO (C), and GFP-tubulin signal in the outer and inner segments (D). (E) Representative ET slice images (lateral view) of the outer segment in wild type (left) and c01236/BE6 (right). Note that in mutant cells, several microtubules (red arrowheads), including a doublet (yellow arrowheads), spanned across the TB and MO. Scale bars, 0.5 µm. (F) Representative ET slice images (cross-sectional view) of the outer segment in c01236/BE6. Note that the number of microtubules in the outer segment was largely reduced. Scale bar, 0.5 µm. (G) Statistical quantification of the ratio between the number of microtubules that spanned across the TB and MO (see Materials and methods) to the number of MO microtubules. (H) Representative confocal images showing Patronin-RFP signals in the leg receptors of wild type (patronin-rfp-KI) and c01236/BE6 (patronin-rfp-KI; c01236/BE6). White arrowhead, the MO. Yellow arrowhead, the proximal end of the TB. Green arrowhead, dendritic inner segment. Scale bar, 5 µm. (I) Statistical quantification of Patronin-RFP signal at the distal region of the inner segment in wild type and c01236/BE6. (J) Representative confocal snapshots of EB1 comets in wild type (left, EB1-tdgfp-KI) and c01236/BE6 (right, EB1-tdgfp-KI; c01236/BE6). Scale bar, 5 µm. Also see Video 2. (K) Statistical quantification of EB1 comet frequency in the inner segment. In B–D, G, I, and K, data are presented as mean ± SD with scattered data points. Two-sided unpaired Student’s t test was used for statistical analysis. *, P < 0.05; ***, P < 0.001. The number of cells is indicated.

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