Figure 5.
Functions of kat-60L1 in haltere receptors. (A) Representative confocal images (top view) showing the membrane, NompC, and tubulin signals in the outer segment. Upper left: dcx-emap-gal4/uas-cd4-tdTom. Middle left: NompC-gfp-KI. Lower left: dcx-emap-gal4/uas-gfp-αtub84B. Upper right: dcx-emap-gal4/uas-cd4-tdTom; c01236/BE6. Middle right: NompC-gfp-KI; c01236/BE6. Lower right: dcx-emap-gal4/uas-gfp-αtub84B; c01236/BE6. Scale bar, 10 µm. (B) Statistical quantification of the maximum cross-sectional area of the outer segment. (C) Statistical quantification of NompC-GFP signal in the MO. In B and C, the number of halteres (30 cells per haltere) is indicated. (D) Statistical quantification of GFP-tubulin signal in the outer segment. Note that 50% of the haltere receptors (n = 3 halteres) in c01236/BE6 had an undetectable signal (A). The rest showed a 44% reduction (wild type: n = 3 halteres, 148 cells; c01236/BE6: n = 3 halteres, 76 cells; P < 0.05). (E) Representative slice images of the sensory cilium from the volume data acquired using serial block-face imaging. Upper panels: wild type. Lower panels: c01236/BE6.(F) Segmented model of the sensory cilium in wild type (left) and c01236/BE6 (right). In E and F, scale bars, 250 nm. Also see Video 3. (G) Reconstructed model of microtubules in the outer segment (c01236/BE6). Blue, the aberrantly long microtubules. Scale bar, 0.5 µm. (H) Total length of microtubules in the haltere receptors of wild-type and c01236/BE6 flies. The number of receptors was indicated. (I) The length of individual microtubules in the outer segment of haltere receptors in wild type and c01236/BE6. The data were from four and two sets of completely reconstructed wild-type and mutant volume data. The number of microtubules is indicated. Two-sided unpaired Student’s t test with Bonferroni correction. (J) Representative confocal images showing Patronin-RFP signal at the proximal end of the MO in haltere receptors in wild type (left, patronin-rfp-KI) and c01236/BE6 (right, patronin-rfp-KI; c01236/BE6). Scale bar, 1 µm. (K) Statistical quantification of Patronin-RFP signals at the proximal end of the MO in haltere receptors. The number of halteres (10 cells per haltere) is indicated. In B–D, H, and K, data are presented as mean ± SD with scattered data points. Two-sided unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. DC, daughter centriole; MC, mother centriole; TZ, transition zone; mem, membrane.

Functions of kat-60L1 in haltere receptors. (A) Representative confocal images (top view) showing the membrane, NompC, and tubulin signals in the outer segment. Upper left: dcx-emap-gal4/uas-cd4-tdTom. Middle left: NompC-gfp-KI. Lower left: dcx-emap-gal4/uas-gfp-αtub84B. Upper right: dcx-emap-gal4/uas-cd4-tdTom; c01236/BE6. Middle right: NompC-gfp-KI; c01236/BE6. Lower right: dcx-emap-gal4/uas-gfp-αtub84B; c01236/BE6. Scale bar, 10 µm. (B) Statistical quantification of the maximum cross-sectional area of the outer segment. (C) Statistical quantification of NompC-GFP signal in the MO. In B and C, the number of halteres (30 cells per haltere) is indicated. (D) Statistical quantification of GFP-tubulin signal in the outer segment. Note that 50% of the haltere receptors (n = 3 halteres) in c01236/BE6 had an undetectable signal (A). The rest showed a 44% reduction (wild type: n = 3 halteres, 148 cells; c01236/BE6: n = 3 halteres, 76 cells; P < 0.05). (E) Representative slice images of the sensory cilium from the volume data acquired using serial block-face imaging. Upper panels: wild type. Lower panels: c01236/BE6.(F) Segmented model of the sensory cilium in wild type (left) and c01236/BE6 (right). In E and F, scale bars, 250 nm. Also see Video 3. (G) Reconstructed model of microtubules in the outer segment (c01236/BE6). Blue, the aberrantly long microtubules. Scale bar, 0.5 µm. (H) Total length of microtubules in the haltere receptors of wild-type and c01236/BE6 flies. The number of receptors was indicated. (I) The length of individual microtubules in the outer segment of haltere receptors in wild type and c01236/BE6. The data were from four and two sets of completely reconstructed wild-type and mutant volume data. The number of microtubules is indicated. Two-sided unpaired Student’s t test with Bonferroni correction. (J) Representative confocal images showing Patronin-RFP signal at the proximal end of the MO in haltere receptors in wild type (left, patronin-rfp-KI) and c01236/BE6 (right, patronin-rfp-KI; c01236/BE6). Scale bar, 1 µm. (K) Statistical quantification of Patronin-RFP signals at the proximal end of the MO in haltere receptors. The number of halteres (10 cells per haltere) is indicated. In B–D, H, and K, data are presented as mean ± SD with scattered data points. Two-sided unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. DC, daughter centriole; MC, mother centriole; TZ, transition zone; mem, membrane.

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