SBP–EGFP–N-cadherin is delivered to the PM by SNAP23, VAMP8, and Stx1B in the COS7 cells. (A) Fluorescence images of total SBP–EGFP–N-cadherin (Ncad; green) and cell surface SBP–EGFP–Ncad labeled by GFP antibody (red) in the control (siLuciferase) and SNAP23-, VAMP8-, or Stx1B-depleted COS7 cells 60 min after biotin treatment. The cells were stained under nonpermeabilized conditions. Scale bars, 5 µm. (B) Fluorescence images of SBP–EGFP–Ncad, mCherry–VAMP8, and LAMP2 (a lysosome marker) in the COS7 cells 60 min after biotin treatment. The depicted cell is the same cell shown in Fig. 9 B. EGFP and mCherry double-positive vesicular structures (blue arrowheads) are not colocalized with LAMP2 (white puncta in the right panels). Lower panels show magnified images of the boxes surrounded by dashed lines in the upper panels. Scale bars, 5 µm. (C) Fluorescence images of SBP–EGFP–Ncad and SBP–mCherry–GPI in the COS7 cells 60 min after biotin treatment. Lower panels show magnified images of the boxes surrounded by dashed lines in the upper panels. Scale bars, 5 µm.