Knockdown of SNAP23 decreases the PM localization of N-cadherin in cultured RGCs. (A) N-cadherin (Ncad; green) and SNAP23 (red) stained with DAPI (blue) in the control (Ctrl; siLuciferase) and SNAP23-depleted RGCs. Scale bar, 10 µm. (B) N-cadherin ectodomain stained in the Ctrl and SNAP23-depleted RGCs. Scale bar, 10 µm. (C and D) Immunoblots showing the levels of total and cell surface N-cadherin, β1-integrin, Ephrin-B1, LDLR, and Na⁺/K⁺-ATPase in the Ctrl and SNAP23-depleted RGCs. Relative expression was calculated from three independent experiments. (E) Diagrams of N-cadherin–mCherry and N-cadherin–LDLR–mCherry. (F and G) SNAP23 and ZO-1 stained in the SNAP23 sgRNA and N-cadherin–mCherry (F) or N-cadherin–LDLR–mCherry (G) electroporated region of the cerebral cortex. An empty pX330 plasmid was electroporated as a control. mCherry staining shows the electroporated region. Lower panels (F′, F′′, G′, and G′′) show magnified images of the boxes surrounded by dashed lines in the upper panels. Arrowheads in G′′ indicate staining of recovered ZO-1. Scale bars, 100 µm. The bar graphs represent the means ± SEM. Significance was calculated using two-tailed paired Student’s t tests. Statistical significance is indicated by *P < 0.05 or **P < 0.01.