Figure 2.
NcKO RGCs lose their apico-basal polarity.(A) Nestin stained in coronal sections of the cerebrum at E13.5. Right panels show magnified images of the boxes surrounded by dashed lines in the left panels. Scale bars, 50 µm. (B) Pericentrin (Pcnt; red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Arrows indicate abnormal localization of basal bodies in RGCs. Scale bar, 20 µm. (C) Pax6 (red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Scale bar, 50 µm. (D) Density of the Pax6-positive cells in the E13.5 cerebral cortex (n = 12 sections from three mice of each genotype). (E) Nestin stained in sagittal sections of the cerebellum at E13.5. Right panels show magnified images of the boxes surrounded by dashed lines in the left panels. Scale bars, 50 µm. (F) Pericentrin (red) and DAPI (blue) stained in sagittal sections of the cerebellum at E13.5. Arrows indicate the abnormal localization of the basal bodies in RGCs. Scale bar, 20 µm. (G) Pax6 (red) and DAPI (blue) stained in sagittal sections of the cerebellum at E13.5. Scale bar, 50 µm. (H) Number of Pax6-positive cells in the rhombic lip (RL) area in cerebellar sections (n = 3 sections from three mice per genotype). (I) BrdU (red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Scale bar, 50 µm. (J) Distributions of BrdU-positive S-phase RGCs in cerebral sections (n = 5 sections from three mice per genotype). (K) PH3 (red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Scale bar, 50 µm. (L) Distributions of PH3-positive M-phase RGCs in cerebral sections (n = 5 sections from three mice per genotype). The box plots represent the maximum and minimum values. The centerlines of the box plots represent the medians of the data. The bar graphs represent the means ± SEM. Significance was calculated by using two-tailed paired Student’s t tests. Statistical significance is indicated by *P < 0.05, **P < 0.01, or ***P < 0.001. Ctrl, control.

NcKO RGCs lose their apico-basal polarity. (A) Nestin stained in coronal sections of the cerebrum at E13.5. Right panels show magnified images of the boxes surrounded by dashed lines in the left panels. Scale bars, 50 µm. (B) Pericentrin (Pcnt; red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Arrows indicate abnormal localization of basal bodies in RGCs. Scale bar, 20 µm. (C) Pax6 (red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Scale bar, 50 µm. (D) Density of the Pax6-positive cells in the E13.5 cerebral cortex (n = 12 sections from three mice of each genotype). (E) Nestin stained in sagittal sections of the cerebellum at E13.5. Right panels show magnified images of the boxes surrounded by dashed lines in the left panels. Scale bars, 50 µm. (F) Pericentrin (red) and DAPI (blue) stained in sagittal sections of the cerebellum at E13.5. Arrows indicate the abnormal localization of the basal bodies in RGCs. Scale bar, 20 µm. (G) Pax6 (red) and DAPI (blue) stained in sagittal sections of the cerebellum at E13.5. Scale bar, 50 µm. (H) Number of Pax6-positive cells in the rhombic lip (RL) area in cerebellar sections (n = 3 sections from three mice per genotype). (I) BrdU (red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Scale bar, 50 µm. (J) Distributions of BrdU-positive S-phase RGCs in cerebral sections (n = 5 sections from three mice per genotype). (K) PH3 (red) and DAPI (blue) stained in coronal sections of the cerebrum at E13.5. Scale bar, 50 µm. (L) Distributions of PH3-positive M-phase RGCs in cerebral sections (n = 5 sections from three mice per genotype). The box plots represent the maximum and minimum values. The centerlines of the box plots represent the medians of the data. The bar graphs represent the means ± SEM. Significance was calculated by using two-tailed paired Student’s t tests. Statistical significance is indicated by *P < 0.05, **P < 0.01, or ***P < 0.001. Ctrl, control.

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