Localization of SNAP23, VAMP8, and Stx1B in the cerebral cortex of WT mice at E13.5. (A) Nestin (green) and SNAP23 (upper, red), VAMP8 (middle, red), or Stx1B (lower, red) stained in the cerebral cortex of the WT mice at E13.5. Arrows indicate the apical side of the RGCs. Scale bars, 50 µm. (B) SNAP23 (green) and SNAP25 (red) stained in the cerebral cortex of the WT mice at E13.5. SNAP23 preferentially localizes to the apical side of the RGCs (arrows), whereas SNAP25 localizes to the pial side. Scale bar, 50 µm. (C) N-cadherin (N-cad; green) and SNAP23 (upper, red), VAMP8 (middle, red), or Stx1B (lower, red) stained in the cerebral cortex of the WT mice at E13.5. Scale bars, 20 µm. These SNARE components and N-cadherin are partially colocalized to the apical side in RGCs. (D) Pals1 (green) and SNAP23 (red) stained in the cerebral cortex of the WT mice at E13.5. Scale bars, 20 µm. More Pals1 than SNAP23 localize to the apical side of the RGCs. (E) Pals1 (green) and Crb3 (red) stained in the cerebral cortex of the WT mice at E13.5. Scale bars, 20 µm. Crb3 and Pals1 are colocalized at the apical surface of the RGCs.