Degradation analysis of Ypq1 TM5 and TM7 Ala scanning mutants. Related to Fig. 3. (A–C) Heat map showing the degradation defect of all Ypq1 mutants. Mutants with at least 80% (*) or 90% (**) FR scores are highlighted. (D) Top: Degradation of Ypq1 TM5 Ala mutants before (0 h) or after (6 h) Lys starvation. WT and L218A are nonblocking controls. Bottom: Quantification (±SD, n = 2). (E) Top: Degradation of Ypq1 TM7 Ala mutants before (0 h) or after (6 h) Lys starvation. WT and F287A are nonblocking controls. Bottom: Quantification (±SD, n = 2).