CoIP of Ypq1 and Ssh4 in the presence of an Rsp5 mutant. Related to Fig. 2. (A) Degradation of Ypq1-GFP before (0 h) or after (6 h) Lys starvation in the presence of WT Rsp5 or rsp5^G747E. (B) CoIP of Ypq1-GFP with WT Ssh4 (bait) expressed under its native promoter (P^SSH4) or an overexpression promoter (P^CYC1) in a weak Rsp5 mutant (rsp5^G747E) background. IP, immunoprecipitation.