Figure S3.
PREM of a growth cone from a DIV14 hippocampal neuron stained with secondary 18-nm gold-conjugated anti-rabbit antibody without primary antibodies. Boxed region in A is enlarged in B. No gold particles can be detected in these conditions confirming specificity of immunogold stainings. Red arrows indicate microtubules; magenta arrows indicate microtubule tips. Scale bars, 2 µm (A) and 200 nm (B).