Figure S2.
Localization of APC in growth cones of rat hippocampal neurons treated with low concentrations of actin polymerization inhibitors. (A and B) Fluorescence staining with phalloidin (F-actin) and antibodies to APC and βIII tubulin (Tuj1) of DIV3 neurons treated with 1 µM LatB (A) or 1 µM CytoD (B) for the last 24 h of culture. Two examples are shown in each case. (C and D) Immunogold PREM with APC antibody and 12-nm colloidal gold-conjugated secondary antibody of DIV2 neurons treated with 1 µM CytoD for the last 24 h of culture. Boxed region in C is enlarged in D. Gold particles are highlighted in yellow. Scale bars, 10 µm (A and B), 2 µm (C), and 100 nm (D).

Localization of APC in growth cones of rat hippocampal neurons treated with low concentrations of actin polymerization inhibitors. (A and B) Fluorescence staining with phalloidin (F-actin) and antibodies to APC and βIII tubulin (Tuj1) of DIV3 neurons treated with 1 µM LatB (A) or 1 µM CytoD (B) for the last 24 h of culture. Two examples are shown in each case. (C and D) Immunogold PREM with APC antibody and 12-nm colloidal gold-conjugated secondary antibody of DIV2 neurons treated with 1 µM CytoD for the last 24 h of culture. Boxed region in C is enlarged in D. Gold particles are highlighted in yellow. Scale bars, 10 µm (A and B), 2 µm (C), and 100 nm (D).

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