Expression and localization of APC in neurons. (A) Western blot with APC antibody of cell lysates from DIV3, 7, and 13 hippocampal neurons. Tuj1 antibody is used as loading control. (B) Fluorescence staining of young (DIV2) and mature (DIV14) hippocampal neurons with phalloidin (F-actin) and APC and Tuj1 antibodies. Inset in the DIV2 APC panel shows a contrast-enhanced view of the merged APC and Tuj1 signals for the boxed region. (C) Quantification of the APC patterns in DIV2, DIV5, DIV10, and DIV14 neurons; n corresponds to the number of scored growth cones from 17 (DIV2), 10 (DIV5), 8 (DIV10), and 9 (DIV14) cells from two independent experiments; three additional experiments were evaluated qualitatively. Scale bar, 10 µm (applies to all main panels in B) and 2 µm (inset in DIV2/APC panel).