PALM Acquisition and analysis. (A–C) Positional drift correction in x, y, and z axes respectively, as described in the Materials and methods section and Supplemental materials. (D) Detection localization uncertainty histogram for all detections in a typical FOV. (E) Standard error of the mean of the molecular localizations for a typical FOV. (F) Blinking time constant determination. (G) Example of the results of the nuclear masking algorithm.